Microbial species of safety concern in milk from informal processors in Harare, Zimbabwe
In this study, the bacteriological quality and the presence of Staphylococcus aureus, Escherichia coli O157:H7, Bacillus cereus, Salmonella species, and Pseudomonas species were studied in raw milk, cultured milk, milk handlers and packaging containers. A total of 36 samples were collected over 3 mo...
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| Language: | English |
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Academic Journals
2023
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| Online Access: | https://cris.library.msu.ac.zw//handle/11408/5614 https://doi.org/10.5897/AJMR2016.8139 |
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| author | Desmond Tichaona Mugadza Siyangapi Chimuti Nicholas Midzi Patrick Kamau Njage |
| author2 | Department of Food Science and Nutrition, Midlands State University, Gweru, Zimbabwe. |
| author_facet | Department of Food Science and Nutrition, Midlands State University, Gweru, Zimbabwe. Desmond Tichaona Mugadza Siyangapi Chimuti Nicholas Midzi Patrick Kamau Njage |
| author_sort | Desmond Tichaona Mugadza |
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| description | In this study, the bacteriological quality and the presence of Staphylococcus aureus, Escherichia coli O157:H7, Bacillus cereus, Salmonella species, and Pseudomonas species were studied in raw milk, cultured milk, milk handlers and packaging containers. A total of 36 samples were collected over 3 months from three different farmers. Samples were analyzed for means of counts per milliliter of milk for total bacterial count (TBC), total coliform count (TCC), total E. coli count (TEC), S. aureus, B. cereus, Salmonella spp. and Pseudomonas spp. Microbial load ranged between 0.81 and 7.6 log10 cfu/ml for various critical sampling locations. Isolates of E. coli, S. aureus and B. cereus were taken for simple polymerase chain reaction (PCR) to investigate the presence of virulent genes, rfB, sei, and cytK with amplicon sizes of 1.0 kb, 500 bp and 320 bp, respectively. The sei gene was detected in 19% of the samples and 2.8% were found to have the cytK gene. The rfB gene could not be picked in E. coli. The results show poor hygienic practices at the processors and potential risk to the consumers. |
| format | research article |
| id | ir-11408-5614 |
| institution | My University |
| language | English |
| publishDate | 2023 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | ir-11408-56142023-05-05T06:47:55Z Microbial species of safety concern in milk from informal processors in Harare, Zimbabwe Desmond Tichaona Mugadza Siyangapi Chimuti Nicholas Midzi Patrick Kamau Njage Department of Food Science and Nutrition, Midlands State University, Gweru, Zimbabwe. Department of Food and Microbiology, Government Analyst Laboratory, Harare, Zimbabwe Department of Medical Microbiology, College of Health Sciences, University of Zimbabwe, Zimbabwe Department of Food and Microbiology, University of Nairobi, Kenya Fresh milk fermented milk packaging container milk handler pathogens In this study, the bacteriological quality and the presence of Staphylococcus aureus, Escherichia coli O157:H7, Bacillus cereus, Salmonella species, and Pseudomonas species were studied in raw milk, cultured milk, milk handlers and packaging containers. A total of 36 samples were collected over 3 months from three different farmers. Samples were analyzed for means of counts per milliliter of milk for total bacterial count (TBC), total coliform count (TCC), total E. coli count (TEC), S. aureus, B. cereus, Salmonella spp. and Pseudomonas spp. Microbial load ranged between 0.81 and 7.6 log10 cfu/ml for various critical sampling locations. Isolates of E. coli, S. aureus and B. cereus were taken for simple polymerase chain reaction (PCR) to investigate the presence of virulent genes, rfB, sei, and cytK with amplicon sizes of 1.0 kb, 500 bp and 320 bp, respectively. The sei gene was detected in 19% of the samples and 2.8% were found to have the cytK gene. The rfB gene could not be picked in E. coli. The results show poor hygienic practices at the processors and potential risk to the consumers. 10 32 1257 1262 2023-05-05T06:47:55Z 2023-05-05T06:47:55Z 2016-08-28 research article https://cris.library.msu.ac.zw//handle/11408/5614 https://doi.org/10.5897/AJMR2016.8139 en African Journal of Microbiology Research 1996-0808 open Academic Journals |
| spellingShingle | Fresh milk fermented milk packaging container milk handler pathogens Desmond Tichaona Mugadza Siyangapi Chimuti Nicholas Midzi Patrick Kamau Njage Microbial species of safety concern in milk from informal processors in Harare, Zimbabwe |
| title | Microbial species of safety concern in milk from informal processors in Harare, Zimbabwe |
| title_full | Microbial species of safety concern in milk from informal processors in Harare, Zimbabwe |
| title_fullStr | Microbial species of safety concern in milk from informal processors in Harare, Zimbabwe |
| title_full_unstemmed | Microbial species of safety concern in milk from informal processors in Harare, Zimbabwe |
| title_short | Microbial species of safety concern in milk from informal processors in Harare, Zimbabwe |
| title_sort | microbial species of safety concern in milk from informal processors in harare, zimbabwe |
| topic | Fresh milk fermented milk packaging container milk handler pathogens |
| url | https://cris.library.msu.ac.zw//handle/11408/5614 https://doi.org/10.5897/AJMR2016.8139 |
| work_keys_str_mv | AT desmondtichaonamugadza microbialspeciesofsafetyconcerninmilkfrominformalprocessorsinhararezimbabwe AT siyangapichimuti microbialspeciesofsafetyconcerninmilkfrominformalprocessorsinhararezimbabwe AT nicholasmidzi microbialspeciesofsafetyconcerninmilkfrominformalprocessorsinhararezimbabwe AT patrickkamaunjage microbialspeciesofsafetyconcerninmilkfrominformalprocessorsinhararezimbabwe |