Tracing cross species transmission of Mycobacterium bovis at the wildlife/ livestock interface in South Africa
Background: Bovine tuberculosis (bTB) affects cattle and wildlife in South Africa with the African buffalo (Syncerus caffer) as the principal maintenance host. The presence of a wildlife maintenance host at the wildlife/livestock interface acting as spill-over host makes it much more challenging to...
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| Language: | English |
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BioMed Central Ltd.
2021
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| Online Access: | http://hdl.handle.net/11408/4233 |
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| author | Sichewo, Petronillah R. Hlokwe, Tiny M. Etter, Eric M. C. Miche, Anita L. |
| author_facet | Sichewo, Petronillah R. Hlokwe, Tiny M. Etter, Eric M. C. Miche, Anita L. |
| author_sort | Sichewo, Petronillah R. |
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| description | Background: Bovine tuberculosis (bTB) affects cattle and wildlife in South Africa with the African buffalo (Syncerus caffer) as the principal maintenance host. The presence of a wildlife maintenance host at the wildlife/livestock interface acting as spill-over host makes it much more challenging to control and eradicate bTB in cattle.
Spoligotyping and mycobacterial interspersed repetitive unit-variable number of tandem repeat (MIRU-VNTR) genotyping methods were performed to investigate the genetic diversity of Mycobacterium bovis (M. bovis) isolates from cattle and wildlife, their distribution and transmission at the wildlife/livestock interface in northern Kwa-Zulu Natal (KZN), South Africa.
Results: SB0130 was identified as the dominant spoligotype pattern at this wildlife/livestock interface, while VNTR typing revealed a total of 29 VNTR profiles (strains) in the KZN province signifying high genetic variability. The detection of 5 VNTR profiles shared between cattle and buffalo suggests M. bovis transmission between species.
MIRU-VNTR confirmed co-infection in one cow with three strains of M. bovis that differed at a single locus, with 2 being shared with buffalo, implying pathogen introduction from most probably unrelated wildlife sources.
Conclusion: Our findings highlight inter and intra species transmission of bTB at the wildlife/livestock interface and the need for the implementation of adequate bTB control measures to mitigate the spread of the pathogen responsible for economic losses and a public health threat. |
| format | Article |
| id | ir-11408-4233 |
| institution | My University |
| language | English |
| publishDate | 2021 |
| publisher | BioMed Central Ltd. |
| record_format | dspace |
| spelling | ir-11408-42332022-06-27T13:49:06Z Tracing cross species transmission of Mycobacterium bovis at the wildlife/ livestock interface in South Africa Sichewo, Petronillah R. Hlokwe, Tiny M. Etter, Eric M. C. Miche, Anita L. African buffalo Bovine tuberculosis (bTB) Mycobacterium bovis (M. bovis) Wildlife/ livestock interface strains wildlife Background: Bovine tuberculosis (bTB) affects cattle and wildlife in South Africa with the African buffalo (Syncerus caffer) as the principal maintenance host. The presence of a wildlife maintenance host at the wildlife/livestock interface acting as spill-over host makes it much more challenging to control and eradicate bTB in cattle. Spoligotyping and mycobacterial interspersed repetitive unit-variable number of tandem repeat (MIRU-VNTR) genotyping methods were performed to investigate the genetic diversity of Mycobacterium bovis (M. bovis) isolates from cattle and wildlife, their distribution and transmission at the wildlife/livestock interface in northern Kwa-Zulu Natal (KZN), South Africa. Results: SB0130 was identified as the dominant spoligotype pattern at this wildlife/livestock interface, while VNTR typing revealed a total of 29 VNTR profiles (strains) in the KZN province signifying high genetic variability. The detection of 5 VNTR profiles shared between cattle and buffalo suggests M. bovis transmission between species. MIRU-VNTR confirmed co-infection in one cow with three strains of M. bovis that differed at a single locus, with 2 being shared with buffalo, implying pathogen introduction from most probably unrelated wildlife sources. Conclusion: Our findings highlight inter and intra species transmission of bTB at the wildlife/livestock interface and the need for the implementation of adequate bTB control measures to mitigate the spread of the pathogen responsible for economic losses and a public health threat. 2021-05-24T07:27:33Z 2021-05-24T07:27:33Z 2020 Article 1471-2180 http://hdl.handle.net/11408/4233 en BMC Microbiology;Vol.20 ; No.49 open BioMed Central Ltd. |
| spellingShingle | African buffalo Bovine tuberculosis (bTB) Mycobacterium bovis (M. bovis) Wildlife/ livestock interface strains wildlife Sichewo, Petronillah R. Hlokwe, Tiny M. Etter, Eric M. C. Miche, Anita L. Tracing cross species transmission of Mycobacterium bovis at the wildlife/ livestock interface in South Africa |
| title | Tracing cross species transmission of Mycobacterium bovis at the wildlife/ livestock interface in South Africa |
| title_full | Tracing cross species transmission of Mycobacterium bovis at the wildlife/ livestock interface in South Africa |
| title_fullStr | Tracing cross species transmission of Mycobacterium bovis at the wildlife/ livestock interface in South Africa |
| title_full_unstemmed | Tracing cross species transmission of Mycobacterium bovis at the wildlife/ livestock interface in South Africa |
| title_short | Tracing cross species transmission of Mycobacterium bovis at the wildlife/ livestock interface in South Africa |
| title_sort | tracing cross species transmission of mycobacterium bovis at the wildlife/ livestock interface in south africa |
| topic | African buffalo Bovine tuberculosis (bTB) Mycobacterium bovis (M. bovis) Wildlife/ livestock interface strains wildlife |
| url | http://hdl.handle.net/11408/4233 |
| work_keys_str_mv | AT sichewopetronillahr tracingcrossspeciestransmissionofmycobacteriumbovisatthewildlifelivestockinterfaceinsouthafrica AT hlokwetinym tracingcrossspeciestransmissionofmycobacteriumbovisatthewildlifelivestockinterfaceinsouthafrica AT etterericmc tracingcrossspeciestransmissionofmycobacteriumbovisatthewildlifelivestockinterfaceinsouthafrica AT micheanital tracingcrossspeciestransmissionofmycobacteriumbovisatthewildlifelivestockinterfaceinsouthafrica |