Antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in Harare, Zimbabwe
This study aimed to: (1) assess Escherichia coli contamination in polony, beef burgers and traditionally fermented cow milk from the formal and informal markets in Harare, Zimbabwe, (2) determine the antibiotic sensitivity of Escherichia coli isolates, and (3) identify Shiga-toxin producing Escheric...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2021
|
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/antimicrobial-resistant-shiga-toxin-producing-escherichia-coli-isolated-from-ready-to-eat-meat-products-and-fermented-milk-sold-in-the-formal-and-informal-sectors-in-harare-zimbabwe/ http://hdl.handle.net/11408/4189 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1779905266001641472 |
|---|---|
| author | Gweshe, Wardner Munashe Muteveri, Tinashe Gufe, Claudious Marumure, Jerikias Hodobo, Tinashe Canaan |
| author_facet | Gweshe, Wardner Munashe Muteveri, Tinashe Gufe, Claudious Marumure, Jerikias Hodobo, Tinashe Canaan |
| author_sort | Gweshe, Wardner Munashe |
| collection | DSpace |
| description | This study aimed to: (1) assess Escherichia coli contamination in polony, beef burgers and traditionally fermented cow milk from the formal and informal markets in Harare, Zimbabwe, (2) determine the antibiotic sensitivity of Escherichia coli isolates, and (3) identify Shiga-toxin producing Escherichia coli isolates using the presence of virulence genes, namely, intimin, enterohemolysin A and Shiga toxins 1 and 2. Ninety-six samples comprising 32 beef polony slices, 32 beef burger patties, and 32 fermented milk specimens were obtained from the informal and formal outlets of the central business district. Escherichia coli occurred in 20 (21%) of the samples, being more prevalent in the informal (29%) than
in the formal (13%) market. Of the 20 E. coli isolates, 6 (30%) were Shiga-toxin producing E. coli, and the rest (70%) were negative for virulence genes. The predominance of Escherichia coli was greater in meat products (25%) than in fermented milk (13%). Total Escherichia coli counts were not substantially
different between formal and informal markets (t-test: p=0.08). All the E. coli isolates were multidrugresistant with antimicrobial resistance prevalence ranging from 25% for Sulphamethoxazole to 100% for Penicillin and Erythromycin. The presence of E. coli in food indicates faecal contamination and probable existence of other enteric pathogens. The presence of virulent and antimicrobial-resistant
E. coli strains in food threatens food safety and public health. We conclude that ready-to-eat animal products from both informal and formal sectors could result in the dissemination of antimicrobialresistant Escherichia coli species if corrective measures are not taken. |
| format | Article |
| id | ir-11408-4189 |
| institution | My University |
| language | English |
| publishDate | 2021 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | dspace |
| spelling | ir-11408-41892022-06-27T13:49:06Z Antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in Harare, Zimbabwe Gweshe, Wardner Munashe Muteveri, Tinashe Gufe, Claudious Marumure, Jerikias Hodobo, Tinashe Canaan Shiga-toxin producing E.coli Escherichia coli ready-to-eat animal products antimicrobial resistance This study aimed to: (1) assess Escherichia coli contamination in polony, beef burgers and traditionally fermented cow milk from the formal and informal markets in Harare, Zimbabwe, (2) determine the antibiotic sensitivity of Escherichia coli isolates, and (3) identify Shiga-toxin producing Escherichia coli isolates using the presence of virulence genes, namely, intimin, enterohemolysin A and Shiga toxins 1 and 2. Ninety-six samples comprising 32 beef polony slices, 32 beef burger patties, and 32 fermented milk specimens were obtained from the informal and formal outlets of the central business district. Escherichia coli occurred in 20 (21%) of the samples, being more prevalent in the informal (29%) than in the formal (13%) market. Of the 20 E. coli isolates, 6 (30%) were Shiga-toxin producing E. coli, and the rest (70%) were negative for virulence genes. The predominance of Escherichia coli was greater in meat products (25%) than in fermented milk (13%). Total Escherichia coli counts were not substantially different between formal and informal markets (t-test: p=0.08). All the E. coli isolates were multidrugresistant with antimicrobial resistance prevalence ranging from 25% for Sulphamethoxazole to 100% for Penicillin and Erythromycin. The presence of E. coli in food indicates faecal contamination and probable existence of other enteric pathogens. The presence of virulent and antimicrobial-resistant E. coli strains in food threatens food safety and public health. We conclude that ready-to-eat animal products from both informal and formal sectors could result in the dissemination of antimicrobialresistant Escherichia coli species if corrective measures are not taken. 2021-05-18T08:44:36Z 2021-05-18T08:44:36Z 2020 Article 2581-690X https://microbiologyjournal.org/antimicrobial-resistant-shiga-toxin-producing-escherichia-coli-isolated-from-ready-to-eat-meat-products-and-fermented-milk-sold-in-the-formal-and-informal-sectors-in-harare-zimbabwe/ http://hdl.handle.net/11408/4189 en Journal of Pure and Applied Microbiology;Vol.14 ; No.2 : p.1157-1165 open Journal of Pure and Applied Microbiology |
| spellingShingle | Shiga-toxin producing E.coli Escherichia coli ready-to-eat animal products antimicrobial resistance Gweshe, Wardner Munashe Muteveri, Tinashe Gufe, Claudious Marumure, Jerikias Hodobo, Tinashe Canaan Antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in Harare, Zimbabwe |
| title | Antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in Harare, Zimbabwe |
| title_full | Antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in Harare, Zimbabwe |
| title_fullStr | Antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in Harare, Zimbabwe |
| title_full_unstemmed | Antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in Harare, Zimbabwe |
| title_short | Antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in Harare, Zimbabwe |
| title_sort | antimicrobial-resistant shiga-toxin producing escherichia coli isolated from ready-to-eat meat products and fermented milk sold in the formal and informal sectors in harare, zimbabwe |
| topic | Shiga-toxin producing E.coli Escherichia coli ready-to-eat animal products antimicrobial resistance |
| url | https://microbiologyjournal.org/antimicrobial-resistant-shiga-toxin-producing-escherichia-coli-isolated-from-ready-to-eat-meat-products-and-fermented-milk-sold-in-the-formal-and-informal-sectors-in-harare-zimbabwe/ http://hdl.handle.net/11408/4189 |
| work_keys_str_mv | AT gweshewardnermunashe antimicrobialresistantshigatoxinproducingescherichiacoliisolatedfromreadytoeatmeatproductsandfermentedmilksoldintheformalandinformalsectorsinhararezimbabwe AT muteveritinashe antimicrobialresistantshigatoxinproducingescherichiacoliisolatedfromreadytoeatmeatproductsandfermentedmilksoldintheformalandinformalsectorsinhararezimbabwe AT gufeclaudious antimicrobialresistantshigatoxinproducingescherichiacoliisolatedfromreadytoeatmeatproductsandfermentedmilksoldintheformalandinformalsectorsinhararezimbabwe AT marumurejerikias antimicrobialresistantshigatoxinproducingescherichiacoliisolatedfromreadytoeatmeatproductsandfermentedmilksoldintheformalandinformalsectorsinhararezimbabwe AT hodobotinashecanaan antimicrobialresistantshigatoxinproducingescherichiacoliisolatedfromreadytoeatmeatproductsandfermentedmilksoldintheformalandinformalsectorsinhararezimbabwe |