Induction of anti-tumor immunity by human telomerase reverse transcriptase (hTERT) and viral-derived peptides in breast cancer mouse models
Human telomerase reverse transcriptase (hTERT) is the most common tumor-associated antigen (TAA) and its activity is upregulated in more than 90% of cancers. Therefore, its targeting can be used in cancer immunotherapy. In continuing with our previous study, we evaluated hTERT derived peptides conju...
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| Language: | English |
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2023
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| Online Access: | https://cris.library.msu.ac.zw//handle/11408/5646 https://doi.org/10.21203/rs.3.rs-2751351/v1 |
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| author | Fanuel Songwe Gholam Ali Kardar Abasalt Hosseinzadeh Colagar Mehdi Ghorbani Amir Mohammadi |
| author2 | Department of Biological Sciences, Faculty of Science and Technology Midlands State University Gweru, Zimbabwe |
| author_facet | Department of Biological Sciences, Faculty of Science and Technology Midlands State University Gweru, Zimbabwe Fanuel Songwe Gholam Ali Kardar Abasalt Hosseinzadeh Colagar Mehdi Ghorbani Amir Mohammadi |
| author_sort | Fanuel Songwe |
| collection | DSpace |
| description | Human telomerase reverse transcriptase (hTERT) is the most common tumor-associated antigen (TAA) and its activity is upregulated in more than 90% of cancers. Therefore, its targeting can be used in cancer immunotherapy. In continuing with our previous study, we evaluated hTERT derived peptides conjugated to KLH in mice breast cancer models. To induce tumors in Balb/C mice, about 5 × 10 5 mice breast cancer 4T1 cell were washed and suspended in 100 µl sterile PBS and subcutaneously (s.c) injected to 4th mammary fat pat of 6–8 weeks old mice. Thereafter, tumor size measurements were taken two times a week. About 100 ug KLH conjugated peptides accompanied by Incomplete Freund′s adjuvant (IFA) were injected s.c on days 10th and 20th after cancer cells inoculation. In spite of their significantly lower immunogenicity and affinities on mice HLA compared to human (approximately 5–30 times), these peptides inhibited tumor growth as well as being able to increase the overall survival of vaccinated Balb/C mice compare untreated group. |
| format | research article |
| id | ir-11408-5646 |
| institution | My University |
| language | English |
| publishDate | 2023 |
| publisher | Research Square |
| record_format | dspace |
| spelling | ir-11408-56462023-05-20T12:05:31Z Induction of anti-tumor immunity by human telomerase reverse transcriptase (hTERT) and viral-derived peptides in breast cancer mouse models Fanuel Songwe Gholam Ali Kardar Abasalt Hosseinzadeh Colagar Mehdi Ghorbani Amir Mohammadi Department of Biological Sciences, Faculty of Science and Technology Midlands State University Gweru, Zimbabwe Tehran University of Medical Sciences University of Mazandaran Tehran University of Medical Science University of Mazandaran hTERT MHC Peptide-based vaccine Viral peptide Cancer immunotherapy Human telomerase reverse transcriptase (hTERT) is the most common tumor-associated antigen (TAA) and its activity is upregulated in more than 90% of cancers. Therefore, its targeting can be used in cancer immunotherapy. In continuing with our previous study, we evaluated hTERT derived peptides conjugated to KLH in mice breast cancer models. To induce tumors in Balb/C mice, about 5 × 10 5 mice breast cancer 4T1 cell were washed and suspended in 100 µl sterile PBS and subcutaneously (s.c) injected to 4th mammary fat pat of 6–8 weeks old mice. Thereafter, tumor size measurements were taken two times a week. About 100 ug KLH conjugated peptides accompanied by Incomplete Freund′s adjuvant (IFA) were injected s.c on days 10th and 20th after cancer cells inoculation. In spite of their significantly lower immunogenicity and affinities on mice HLA compared to human (approximately 5–30 times), these peptides inhibited tumor growth as well as being able to increase the overall survival of vaccinated Balb/C mice compare untreated group. 1 17 2023-05-20T12:05:30Z 2023-05-20T12:05:30Z 2023-04-04 research article https://cris.library.msu.ac.zw//handle/11408/5646 https://doi.org/10.21203/rs.3.rs-2751351/v1 en open Research Square |
| spellingShingle | hTERT MHC Peptide-based vaccine Viral peptide Cancer immunotherapy Fanuel Songwe Gholam Ali Kardar Abasalt Hosseinzadeh Colagar Mehdi Ghorbani Amir Mohammadi Induction of anti-tumor immunity by human telomerase reverse transcriptase (hTERT) and viral-derived peptides in breast cancer mouse models |
| title | Induction of anti-tumor immunity by human telomerase reverse transcriptase (hTERT) and viral-derived peptides in breast cancer mouse models |
| title_full | Induction of anti-tumor immunity by human telomerase reverse transcriptase (hTERT) and viral-derived peptides in breast cancer mouse models |
| title_fullStr | Induction of anti-tumor immunity by human telomerase reverse transcriptase (hTERT) and viral-derived peptides in breast cancer mouse models |
| title_full_unstemmed | Induction of anti-tumor immunity by human telomerase reverse transcriptase (hTERT) and viral-derived peptides in breast cancer mouse models |
| title_short | Induction of anti-tumor immunity by human telomerase reverse transcriptase (hTERT) and viral-derived peptides in breast cancer mouse models |
| title_sort | induction of anti-tumor immunity by human telomerase reverse transcriptase (htert) and viral-derived peptides in breast cancer mouse models |
| topic | hTERT MHC Peptide-based vaccine Viral peptide Cancer immunotherapy |
| url | https://cris.library.msu.ac.zw//handle/11408/5646 https://doi.org/10.21203/rs.3.rs-2751351/v1 |
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