Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review
Background Traditional diagnostic tests for schistosome infections are suboptimal, particularly when the parasite burden is low. In the present review we sought to identify recombinant proteins, peptides, and chimeric proteins with potential to be used as sensitive and specific diagnostic tools f...
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| Format: | research article |
| Language: | English |
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Public Library of Science
2023
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| Online Access: | https://cris.library.msu.ac.zw//handle/11408/5578 https://doi.org/10.1371/journal.pone.0282233 |
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| author | Arthur Vengesai Victor Muleya Herald Midzi Tryphine Vimbai Tinago Isaac Chipako Marble Manuwa Thajasvarie Naicker Takafira Mduluza |
| author2 | Wannaporn Ittiprasert |
| author_facet | Wannaporn Ittiprasert Arthur Vengesai Victor Muleya Herald Midzi Tryphine Vimbai Tinago Isaac Chipako Marble Manuwa Thajasvarie Naicker Takafira Mduluza |
| author_sort | Arthur Vengesai |
| collection | DSpace |
| description | Background
Traditional diagnostic tests for schistosome infections are suboptimal, particularly when the parasite burden is low. In the present review we sought to identify recombinant proteins, peptides, and chimeric proteins with potential to be used as sensitive and specific diagnostic tools for schistosomiasis.
Methods
The review was guided by PRISMA-ScR guidelines, Arksey and O’Malley’s framework, and guidelines from the Joanna Briggs Institute. Five databases were searched: Cochrane library, PubMed, EMBASE, PsycInfo and CINAHL, alongside preprints. Identified literature were assessed by two reviewers for inclusion. A narrative summary was used to interpret the tabulated results.
Results
Diagnostic performances were reported as specificities, sensitivities, and AUC. The AUC for S. haematobium recombinant antigens ranged from 0.65 to 0.98, and 0.69 to 0.96 for urine IgG ELISA. S. mansoni recombinant antigens had sensitivities ranging from 65.3% to 100% and specificities ranging from 57.4% to 100%. Except for 4 peptides which had poor diagnostic performances, most peptides had sensitivities ranging from 67.71% to 96.15% and specificities ranging from 69.23% to 100%. S. mansoni chimeric protein was reported to have a sensitivity of 86.8% and a specificity of 94.2%.
Conclusion
The tetraspanin CD63 antigen had the best diagnostic performance for S. haematobium. The tetraspanin CD63 antigen Serum IgG POC-ICTs had a sensitivity of 89% and a specificity of 100%. Peptide Smp_150390.1 (216–230) serum based IgG ELISA had the best diagnostic performance for S. mansoni with a sensitivity of 96.15% and a specificity of 100%. Peptides were reported to demonstrate good to excellent diagnostic performances. S. mansoni multi-peptide chimeric protein further improved the diagnostic accuracy of synthetic peptides. Together with the advantages associated with urine sampling technique, we recommend development of multi-peptide chimeric proteins urine based point of care tools. |
| format | research article |
| id | ir-11408-5578 |
| institution | My University |
| language | English |
| publishDate | 2023 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | ir-11408-55782023-05-03T07:20:13Z Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review Arthur Vengesai Victor Muleya Herald Midzi Tryphine Vimbai Tinago Isaac Chipako Marble Manuwa Thajasvarie Naicker Takafira Mduluza Wannaporn Ittiprasert Department of Biochemistry, Faculty of Medicine and Health Sciences, Midlands State University, Gweru, Zimbabwe Department of Biochemistry, Faculty of Medicine and Health Sciences, Midlands State University, Gweru, Zimbabwe Department of Biochemistry, University of Zimbabwe, Mt Pleasant, Harare, Zimbabwe Department of Biochemistry, University of Zimbabwe, Mt Pleasant, Harare, Zimbabwe Aravas Pharmaceuticals Pvt LTD, Prospect Industrial Area, Harare, Zimbabwe Department of Biochemistry, University of Zimbabwe, Mt Pleasant, Harare, Zimbabwe Discipline of Optics and Imaging, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa Department of Biochemistry, University of Zimbabwe, Mt Pleasant, Harare, Zimbabwe George Washington University, UNITED STATES Diagnostic performances Schistosoma haematobium Schistosoma mansoni Schistosomiasis Background Traditional diagnostic tests for schistosome infections are suboptimal, particularly when the parasite burden is low. In the present review we sought to identify recombinant proteins, peptides, and chimeric proteins with potential to be used as sensitive and specific diagnostic tools for schistosomiasis. Methods The review was guided by PRISMA-ScR guidelines, Arksey and O’Malley’s framework, and guidelines from the Joanna Briggs Institute. Five databases were searched: Cochrane library, PubMed, EMBASE, PsycInfo and CINAHL, alongside preprints. Identified literature were assessed by two reviewers for inclusion. A narrative summary was used to interpret the tabulated results. Results Diagnostic performances were reported as specificities, sensitivities, and AUC. The AUC for S. haematobium recombinant antigens ranged from 0.65 to 0.98, and 0.69 to 0.96 for urine IgG ELISA. S. mansoni recombinant antigens had sensitivities ranging from 65.3% to 100% and specificities ranging from 57.4% to 100%. Except for 4 peptides which had poor diagnostic performances, most peptides had sensitivities ranging from 67.71% to 96.15% and specificities ranging from 69.23% to 100%. S. mansoni chimeric protein was reported to have a sensitivity of 86.8% and a specificity of 94.2%. Conclusion The tetraspanin CD63 antigen had the best diagnostic performance for S. haematobium. The tetraspanin CD63 antigen Serum IgG POC-ICTs had a sensitivity of 89% and a specificity of 100%. Peptide Smp_150390.1 (216–230) serum based IgG ELISA had the best diagnostic performance for S. mansoni with a sensitivity of 96.15% and a specificity of 100%. Peptides were reported to demonstrate good to excellent diagnostic performances. S. mansoni multi-peptide chimeric protein further improved the diagnostic accuracy of synthetic peptides. Together with the advantages associated with urine sampling technique, we recommend development of multi-peptide chimeric proteins urine based point of care tools. 18 3 1 18 2023-05-03T07:20:12Z 2023-05-03T07:20:12Z 2023-03-02 research article https://cris.library.msu.ac.zw//handle/11408/5578 https://doi.org/10.1371/journal.pone.0282233 en PLoS ONE 1932-6203 open Public Library of Science |
| spellingShingle | Diagnostic performances Schistosoma haematobium Schistosoma mansoni Schistosomiasis Arthur Vengesai Victor Muleya Herald Midzi Tryphine Vimbai Tinago Isaac Chipako Marble Manuwa Thajasvarie Naicker Takafira Mduluza Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review |
| title | Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review |
| title_full | Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review |
| title_fullStr | Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review |
| title_full_unstemmed | Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review |
| title_short | Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review |
| title_sort | diagnostic performances of schistosoma haematobium and schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. systematic scoping review |
| topic | Diagnostic performances Schistosoma haematobium Schistosoma mansoni Schistosomiasis |
| url | https://cris.library.msu.ac.zw//handle/11408/5578 https://doi.org/10.1371/journal.pone.0282233 |
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