Detection of mycotoxins in opaque beer production

In Zimbabwe opaque beer is one of the most common alcoholic beverages. This study was aimed at detecting aflatoxins and Ochratoxin A (OTA) in commercial opaque beer brewing. Aflatoxins and OTA are mycotoxins produced by fungi. Maize, sorghum malt and opaque beer samples were used for microbiologi...

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Main Authors: Marume P., Ndlovu N, Manhokwe S, Ndlovu C.
Other Authors: Department of Food Science and Nutrition, Midlands State University, P Bag 9055, Gweru, Zimbabwe.
Format: research article
Language:English
Published: African Journal of Biological Sciences 2023
Subjects:
Online Access:https://cris.library.msu.ac.zw//handle/11408/5505
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author Marume P.
Ndlovu N
Manhokwe S
Ndlovu C.
author2 Department of Food Science and Nutrition, Midlands State University, P Bag 9055, Gweru, Zimbabwe.
author_facet Department of Food Science and Nutrition, Midlands State University, P Bag 9055, Gweru, Zimbabwe.
Marume P.
Ndlovu N
Manhokwe S
Ndlovu C.
author_sort Marume P.
collection DSpace
description In Zimbabwe opaque beer is one of the most common alcoholic beverages. This study was aimed at detecting aflatoxins and Ochratoxin A (OTA) in commercial opaque beer brewing. Aflatoxins and OTA are mycotoxins produced by fungi. Maize, sorghum malt and opaque beer samples were used for microbiological plating and multi mycotoxin extraction. Aflatoxins, OTA, Aspergillus spp, total viable count, coliforms, Lactobacillus spp, Salmonella, Shigella, Staphylococcus aureus were assessed. Mycotoxins were separated using an High Performance Liquid Chromatography (HPLC). Aflatoxins were not detected in all the samples. OTA concentration for malt was 18 μg/kg, 5.2 μg/kg in beer and absent in maize. Fungi identified in maize were Aspergillus flavus, Aspergillus niger, Aspergillus carbonarius, and Aspergillus fumigatus. Malt contained Aspergillus flavus and Aspergillus carbonarius. Fungi observed in beer were Aspergillus flavus and Aspergillus niger. The beer may need heat treatment methods in order to remove the microorganisms identified and prolong shelf life of the product.
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spelling ir-11408-55052023-03-29T07:16:21Z Detection of mycotoxins in opaque beer production Marume P. Ndlovu N Manhokwe S Ndlovu C. Department of Food Science and Nutrition, Midlands State University, P Bag 9055, Gweru, Zimbabwe. Department of Food Science and Nutrition, Midlands State University, P Bag 9055, Gweru, Zimbabwe Department of Food Science and Nutrition, Midlands State University, P Bag 9055, Gweru, Zimbabwe Department of Food Science and Nutrition, Midlands State University, P Bag 9055, Gweru, Zimbabwe Mycotoxins Aflatoxins Ochratoxin A (OTA) Opaque beer Food safety In Zimbabwe opaque beer is one of the most common alcoholic beverages. This study was aimed at detecting aflatoxins and Ochratoxin A (OTA) in commercial opaque beer brewing. Aflatoxins and OTA are mycotoxins produced by fungi. Maize, sorghum malt and opaque beer samples were used for microbiological plating and multi mycotoxin extraction. Aflatoxins, OTA, Aspergillus spp, total viable count, coliforms, Lactobacillus spp, Salmonella, Shigella, Staphylococcus aureus were assessed. Mycotoxins were separated using an High Performance Liquid Chromatography (HPLC). Aflatoxins were not detected in all the samples. OTA concentration for malt was 18 μg/kg, 5.2 μg/kg in beer and absent in maize. Fungi identified in maize were Aspergillus flavus, Aspergillus niger, Aspergillus carbonarius, and Aspergillus fumigatus. Malt contained Aspergillus flavus and Aspergillus carbonarius. Fungi observed in beer were Aspergillus flavus and Aspergillus niger. The beer may need heat treatment methods in order to remove the microorganisms identified and prolong shelf life of the product. 2 4 84 91 2023-03-29T07:16:20Z 2023-03-29T07:16:20Z 2020-10-05 research article https://cris.library.msu.ac.zw//handle/11408/5505 10.33472/AFJBS.2.4.2020.84-91 en African Journal of Biological Sciences (AFJBS) 2663-2187 open African Journal of Biological Sciences
spellingShingle Mycotoxins
Aflatoxins
Ochratoxin A (OTA)
Opaque beer
Food safety
Marume P.
Ndlovu N
Manhokwe S
Ndlovu C.
Detection of mycotoxins in opaque beer production
title Detection of mycotoxins in opaque beer production
title_full Detection of mycotoxins in opaque beer production
title_fullStr Detection of mycotoxins in opaque beer production
title_full_unstemmed Detection of mycotoxins in opaque beer production
title_short Detection of mycotoxins in opaque beer production
title_sort detection of mycotoxins in opaque beer production
topic Mycotoxins
Aflatoxins
Ochratoxin A (OTA)
Opaque beer
Food safety
url https://cris.library.msu.ac.zw//handle/11408/5505
work_keys_str_mv AT marumep detectionofmycotoxinsinopaquebeerproduction
AT ndlovun detectionofmycotoxinsinopaquebeerproduction
AT manhokwes detectionofmycotoxinsinopaquebeerproduction
AT ndlovuc detectionofmycotoxinsinopaquebeerproduction