Aquaporin 5 Expression and Its Relationship to Apoptosis in Different Grades of Differentiated Non-Small Cell Lung Carcinoma
Aquaporin 5 has been recently found as an important oncogenic marker whose expression levels seem to be determined by the level of cellular differentiation. Despite aquaporin volume decrease (AVD) being the most conserved earliest event in apoptosis, there is still a paucity of studies exploring on...
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Scientific Research Publishing
2022
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| author | Fidelis Chibhabha Yaqiu Li Yanyong Hao Hui Zhao Liming Hao |
| author2 | Department of Histology and Embryology, School of Basic Medical Sciences, Norman Bethune College of Medicine, Jilin University, Changchun, China; Department of Anatomy, Faculty of Medicine, Midlands State University, Gweru, Zimbabwe |
| author_facet | Department of Histology and Embryology, School of Basic Medical Sciences, Norman Bethune College of Medicine, Jilin University, Changchun, China; Department of Anatomy, Faculty of Medicine, Midlands State University, Gweru, Zimbabwe Fidelis Chibhabha Yaqiu Li Yanyong Hao Hui Zhao Liming Hao |
| author_sort | Fidelis Chibhabha |
| collection | DSpace |
| description | Aquaporin 5 has been recently found as an important oncogenic marker whose expression levels seem to be determined by the level of cellular differentiation. Despite aquaporin volume decrease (AVD) being the most conserved earliest event in apoptosis, there is still a paucity of studies exploring on aquaporin expression and its relationship with apoptosis in cancer. The aim of this study was to investigate the expression of aquaporin 5 channel protein and to explore on its relationship with apoptosis in well and poorly differentiated non-small cell lung carcinoma both in-vivo and in-vitro. Findings from the study showed that the expression of AQP5 both in-vivo and in-vitro was dependent on the type and degree of tumour differentiation. In-vivo, an increase in aquaporin 5 expression was associated with an increased apoptosis in both poorly and highly differentiated adenocarcinoma (AC) while there was no association between aquaporin 5 expression and apoptosis in both poorly and highly differentiated squamous cell carcinoma (SCC). In vitro, differentiation therapy in the form of ATRA decreased both cell proliferation and increased the expression of AQP5 in A549 cells. The cytomorphological changes, expression of differentiation markers and flow cytometry apoptotic results were dependent on the dose of ATRA treatment. In conclusion, a higher expression of aquaporin 5 was found to promote the rate of the apoptotic process in lung adenocarcinoma (AC). |
| format | research article |
| id | ir-11408-5328 |
| institution | My University |
| language | English |
| publishDate | 2022 |
| publisher | Scientific Research Publishing |
| record_format | dspace |
| spelling | ir-11408-53282022-12-20T14:52:45Z Aquaporin 5 Expression and Its Relationship to Apoptosis in Different Grades of Differentiated Non-Small Cell Lung Carcinoma Fidelis Chibhabha Yaqiu Li Yanyong Hao Hui Zhao Liming Hao Department of Histology and Embryology, School of Basic Medical Sciences, Norman Bethune College of Medicine, Jilin University, Changchun, China; Department of Anatomy, Faculty of Medicine, Midlands State University, Gweru, Zimbabwe Department of Histology and Embryology, School of Basic Medical Sciences, Norman Bethune College of Medicine, Jilin University, Changchun, China; Department of Human Anatomy, Jilin Medical College, Jilin City, China. Department of Pathology, Jilin Province Cancer Hospital, Changchun, China. Department of Histology and Embryology, School of Basic Medical Sciences, Norman Bethune College of Medicine, Jilin University, Changchun, China. Department of Histology and Embryology, School of Basic Medical Sciences, Norman Bethune College of Medicine, Jilin University, Changchun, China. Aquaporin 5 Non-Small Cell Carcinoma Apoptosis Apoptotic Index Aquaporin 5 has been recently found as an important oncogenic marker whose expression levels seem to be determined by the level of cellular differentiation. Despite aquaporin volume decrease (AVD) being the most conserved earliest event in apoptosis, there is still a paucity of studies exploring on aquaporin expression and its relationship with apoptosis in cancer. The aim of this study was to investigate the expression of aquaporin 5 channel protein and to explore on its relationship with apoptosis in well and poorly differentiated non-small cell lung carcinoma both in-vivo and in-vitro. Findings from the study showed that the expression of AQP5 both in-vivo and in-vitro was dependent on the type and degree of tumour differentiation. In-vivo, an increase in aquaporin 5 expression was associated with an increased apoptosis in both poorly and highly differentiated adenocarcinoma (AC) while there was no association between aquaporin 5 expression and apoptosis in both poorly and highly differentiated squamous cell carcinoma (SCC). In vitro, differentiation therapy in the form of ATRA decreased both cell proliferation and increased the expression of AQP5 in A549 cells. The cytomorphological changes, expression of differentiation markers and flow cytometry apoptotic results were dependent on the dose of ATRA treatment. In conclusion, a higher expression of aquaporin 5 was found to promote the rate of the apoptotic process in lung adenocarcinoma (AC). 5 1 1 12 2022-12-20T14:52:45Z 2022-12-20T14:52:45Z 2016-03-31 research article https://cris.library.msu.ac.zw//handle/11408/5328 10.4236/alc.2016.51001 en #PLACEHOLDER_PARENT_METADATA_VALUE# Scientific and Technological Developing Scheme grant of Jilin Province Advances in Lung Cancer 2169-2726 (201015199). open Scientific Research Publishing |
| spellingShingle | Aquaporin 5 Non-Small Cell Carcinoma Apoptosis Apoptotic Index Fidelis Chibhabha Yaqiu Li Yanyong Hao Hui Zhao Liming Hao Aquaporin 5 Expression and Its Relationship to Apoptosis in Different Grades of Differentiated Non-Small Cell Lung Carcinoma |
| title | Aquaporin 5 Expression and Its Relationship to Apoptosis in Different Grades of Differentiated Non-Small Cell Lung Carcinoma |
| title_full | Aquaporin 5 Expression and Its Relationship to Apoptosis in Different Grades of Differentiated Non-Small Cell Lung Carcinoma |
| title_fullStr | Aquaporin 5 Expression and Its Relationship to Apoptosis in Different Grades of Differentiated Non-Small Cell Lung Carcinoma |
| title_full_unstemmed | Aquaporin 5 Expression and Its Relationship to Apoptosis in Different Grades of Differentiated Non-Small Cell Lung Carcinoma |
| title_short | Aquaporin 5 Expression and Its Relationship to Apoptosis in Different Grades of Differentiated Non-Small Cell Lung Carcinoma |
| title_sort | aquaporin 5 expression and its relationship to apoptosis in different grades of differentiated non-small cell lung carcinoma |
| topic | Aquaporin 5 Non-Small Cell Carcinoma Apoptosis Apoptotic Index |
| url | https://cris.library.msu.ac.zw//handle/11408/5328 |
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