Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2
Analysis of the genome sequence of the potato cyst nematode, Globodera pallida, has shown that a substantial gene family (approximately 300 sequences) of proteins containing a SPRY domain is present in this species. This is a huge expansion of the gene family as compared to other organisms, includin...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Brill Academic Publishers
2021
|
| Subjects: | |
| Online Access: | https://doi.org/10.1163/15685411-00002875 https://brill.com/view/journals/nemy/17/4/article-p409_3.xml?language=en http://hdl.handle.net/11408/4390 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1779905669186453504 |
|---|---|
| author | Mei, Yuanyuan Thorpe, Peter Guzha, Athanas Haegeman, Annelies Blok, Vivian C. MacKenzie, Katrin Gheysen, Godelieve Jones, John T. Mantelin, Sophie |
| author_facet | Mei, Yuanyuan Thorpe, Peter Guzha, Athanas Haegeman, Annelies Blok, Vivian C. MacKenzie, Katrin Gheysen, Godelieve Jones, John T. Mantelin, Sophie |
| author_sort | Mei, Yuanyuan |
| collection | DSpace |
| description | Analysis of the genome sequence of the potato cyst nematode, Globodera pallida, has shown that a substantial gene family (approximately 300 sequences) of proteins containing a SPRY domain is present in this species. This is a huge expansion of the gene family as compared to other organisms, including other plant-parasitic nematodes. Some SPRY domain proteins from G. pallida and G. rostochiensis have signal peptides for secretion and are deployed as effectors. One of these SPRYSEC proteins has been shown to suppress host defence responses. We describe further analysis of this gene family in G. pallida. We show that only a minority (10%) of the SPRY domain proteins in this species have a predicted signal peptide for secretion and that the presence of a signal peptide is strongly correlated with the corresponding gene being expressed at the early stages of parasitism. The data suggest that while the gene family is greatly expanded, only a minority of SPRY domain proteins in G. pallida are SPRYSEC candidate effectors. We show that several new SPRYSECs from G. pallida are expressed in the dorsal gland cell and demonstrate that some, but not all, of the SPRYSECs can suppress the hypersensitive response induced by co-expression of the resistance gene Gpa2 and its cognate avirulence factor RBP-1 in Nicotiana benthamiana. |
| format | Article |
| id | ir-11408-4390 |
| institution | My University |
| language | English |
| publishDate | 2021 |
| publisher | Brill Academic Publishers |
| record_format | dspace |
| spelling | ir-11408-43902022-06-27T13:49:06Z Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2 Mei, Yuanyuan Thorpe, Peter Guzha, Athanas Haegeman, Annelies Blok, Vivian C. MacKenzie, Katrin Gheysen, Godelieve Jones, John T. Mantelin, Sophie Pharyngeal gland Gpa2 gene Hypersensitive response; Analysis of the genome sequence of the potato cyst nematode, Globodera pallida, has shown that a substantial gene family (approximately 300 sequences) of proteins containing a SPRY domain is present in this species. This is a huge expansion of the gene family as compared to other organisms, including other plant-parasitic nematodes. Some SPRY domain proteins from G. pallida and G. rostochiensis have signal peptides for secretion and are deployed as effectors. One of these SPRYSEC proteins has been shown to suppress host defence responses. We describe further analysis of this gene family in G. pallida. We show that only a minority (10%) of the SPRY domain proteins in this species have a predicted signal peptide for secretion and that the presence of a signal peptide is strongly correlated with the corresponding gene being expressed at the early stages of parasitism. The data suggest that while the gene family is greatly expanded, only a minority of SPRY domain proteins in G. pallida are SPRYSEC candidate effectors. We show that several new SPRYSECs from G. pallida are expressed in the dorsal gland cell and demonstrate that some, but not all, of the SPRYSECs can suppress the hypersensitive response induced by co-expression of the resistance gene Gpa2 and its cognate avirulence factor RBP-1 in Nicotiana benthamiana. 2021-06-07T12:20:42Z 2021-06-07T12:20:42Z 2015 Article 1388-5545 1568-5411 https://doi.org/10.1163/15685411-00002875 https://brill.com/view/journals/nemy/17/4/article-p409_3.xml?language=en http://hdl.handle.net/11408/4390 en Nematology;Vol. 17; No. 4:p. 409-424 open Brill Academic Publishers |
| spellingShingle | Pharyngeal gland Gpa2 gene Hypersensitive response; Mei, Yuanyuan Thorpe, Peter Guzha, Athanas Haegeman, Annelies Blok, Vivian C. MacKenzie, Katrin Gheysen, Godelieve Jones, John T. Mantelin, Sophie Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2 |
| title | Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2 |
| title_full | Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2 |
| title_fullStr | Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2 |
| title_full_unstemmed | Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2 |
| title_short | Only a small subset of the SPRY domain gene family in Globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene Gpa2 |
| title_sort | only a small subset of the spry domain gene family in globodera pallida is likely to encode effectors, two of which suppress host defences induced by the potato resistance gene gpa2 |
| topic | Pharyngeal gland Gpa2 gene Hypersensitive response; |
| url | https://doi.org/10.1163/15685411-00002875 https://brill.com/view/journals/nemy/17/4/article-p409_3.xml?language=en http://hdl.handle.net/11408/4390 |
| work_keys_str_mv | AT meiyuanyuan onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 AT thorpepeter onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 AT guzhaathanas onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 AT haegemanannelies onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 AT blokvivianc onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 AT mackenziekatrin onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 AT gheysengodelieve onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 AT jonesjohnt onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 AT mantelinsophie onlyasmallsubsetofthesprydomaingenefamilyingloboderapallidaislikelytoencodeeffectorstwoofwhichsuppresshostdefencesinducedbythepotatoresistancegenegpa2 |