Diversity of bacillus cereus strains in extended shelf life
Characterisation of 49 Bacillus cereus strains obtained from extended shelf life (ESL) milk and filler nozzles was done using (GTG)5 rep PCR fingerprinting, determining the presence of the virulence genes cytK, nheA, cer and hblA, and discrimination of psychrotrophic and mesophilic strains with 16S...
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2020
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| Online Access: | https://doi.org/10.1016/j.idairyj.2017.06.001 http://hdl.handle.net/11408/3787 |
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| author | Mugadza, Desmond T. Buys, Elna M. |
| author_facet | Mugadza, Desmond T. Buys, Elna M. |
| author_sort | Mugadza, Desmond T. |
| collection | DSpace |
| description | Characterisation of 49 Bacillus cereus strains obtained from extended shelf life (ESL) milk and filler nozzles was done using (GTG)5 rep PCR fingerprinting, determining the presence of the virulence genes cytK, nheA, cer and hblA, and discrimination of psychrotrophic and mesophilic strains with 16S rDNA. Fourteen isolates were selected for 16S partial sequencing. Fingerprinting and sequencing showed evidence of filler nozzles contaminating ESL milk despite high heterogeneity existing between the isolates. While there is high prevalence of cer, hblA and nheA; cytK was not widely distributed. There was 100% and 8% prevalence of mesophilic and psychrotrophic signatures, respectively. Despite the large diversity of the B. cereus strains in this study, there is evidence that filler nozzles and raw milk are a source of contamination of B. cereus in ESL milk. |
| format | Article |
| id | ir-11408-3787 |
| institution | My University |
| language | English |
| publishDate | 2020 |
| publisher | Elsevier |
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| spelling | ir-11408-37872022-06-27T13:49:06Z Diversity of bacillus cereus strains in extended shelf life Mugadza, Desmond T. Buys, Elna M. Bacteria Dairy plant Extended shelf life Genes Characterisation of 49 Bacillus cereus strains obtained from extended shelf life (ESL) milk and filler nozzles was done using (GTG)5 rep PCR fingerprinting, determining the presence of the virulence genes cytK, nheA, cer and hblA, and discrimination of psychrotrophic and mesophilic strains with 16S rDNA. Fourteen isolates were selected for 16S partial sequencing. Fingerprinting and sequencing showed evidence of filler nozzles contaminating ESL milk despite high heterogeneity existing between the isolates. While there is high prevalence of cer, hblA and nheA; cytK was not widely distributed. There was 100% and 8% prevalence of mesophilic and psychrotrophic signatures, respectively. Despite the large diversity of the B. cereus strains in this study, there is evidence that filler nozzles and raw milk are a source of contamination of B. cereus in ESL milk. 2020-07-16T10:25:23Z 2020-07-16T10:25:23Z 2017 Article 0958-6946 https://doi.org/10.1016/j.idairyj.2017.06.001 http://hdl.handle.net/11408/3787 en International Dairy Journal;Vol. 73: p. 144-150 open Elsevier |
| spellingShingle | Bacteria Dairy plant Extended shelf life Genes Mugadza, Desmond T. Buys, Elna M. Diversity of bacillus cereus strains in extended shelf life |
| title | Diversity of bacillus cereus strains in extended shelf life |
| title_full | Diversity of bacillus cereus strains in extended shelf life |
| title_fullStr | Diversity of bacillus cereus strains in extended shelf life |
| title_full_unstemmed | Diversity of bacillus cereus strains in extended shelf life |
| title_short | Diversity of bacillus cereus strains in extended shelf life |
| title_sort | diversity of bacillus cereus strains in extended shelf life |
| topic | Bacteria Dairy plant Extended shelf life Genes |
| url | https://doi.org/10.1016/j.idairyj.2017.06.001 http://hdl.handle.net/11408/3787 |
| work_keys_str_mv | AT mugadzadesmondt diversityofbacilluscereusstrainsinextendedshelflife AT buyselnam diversityofbacilluscereusstrainsinextendedshelflife |