Application of recombinant technology in protein investigations
Book Chapter
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| Format: | Book chapter |
| Language: | English |
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iConcept Press
2016
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| Online Access: | https://www.iconceptpress.com/book/protein...and...applications/.../1206000740.pdf http://hdl.handle.net/11408/1690 |
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| _version_ | 1779905652180647936 |
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| author | Wang, Yu Hua Muleya, Victor Irving, Helen R. |
| author_facet | Wang, Yu Hua Muleya, Victor Irving, Helen R. |
| author_sort | Wang, Yu Hua |
| collection | DSpace |
| description | Book Chapter |
| format | Book chapter |
| id | ir-11408-1690 |
| institution | My University |
| language | English |
| publishDate | 2016 |
| publisher | iConcept Press |
| record_format | dspace |
| spelling | ir-11408-16902022-10-15T20:22:12Z Application of recombinant technology in protein investigations Wang, Yu Hua Muleya, Victor Irving, Helen R. Recombinant technology, Protein investigations Book Chapter A major breakthrough of modern biology is the development of recombinant technology which allows specific genes to be cloned and expressed in recombinant or chimeric forms in order to isolate or detect the defined products. Such technology is particularly useful in protein investigation. It not only helps to isolate target proteins from their natural complicated context and identify function, but also provides distinct and detectable means to track the protein activity in vivo. The current chapter covers a number of methodologies to isolate or detect gene products of interest, including recombinant protein purification and analysis, enzymatic reporter assays and fluorescent tag detection. The chapter is formalized with various research aspects, concrete experiments, step-by-step procedures, and notes dealing with possible problems. It is expected that this informative and practical manual will be applicable as a basic guide for readers. 2016-07-18T08:35:25Z 2016-07-18T08:35:25Z 2014 Book chapter 978-1-922227-62-1 https://www.iconceptpress.com/book/protein...and...applications/.../1206000740.pdf http://hdl.handle.net/11408/1690 en Protein Purification and Analysis II: Methods and Applications;Chapter 3, open iConcept Press |
| spellingShingle | Recombinant technology, Protein investigations Wang, Yu Hua Muleya, Victor Irving, Helen R. Application of recombinant technology in protein investigations |
| title | Application of recombinant technology in protein investigations |
| title_full | Application of recombinant technology in protein investigations |
| title_fullStr | Application of recombinant technology in protein investigations |
| title_full_unstemmed | Application of recombinant technology in protein investigations |
| title_short | Application of recombinant technology in protein investigations |
| title_sort | application of recombinant technology in protein investigations |
| topic | Recombinant technology, Protein investigations |
| url | https://www.iconceptpress.com/book/protein...and...applications/.../1206000740.pdf http://hdl.handle.net/11408/1690 |
| work_keys_str_mv | AT wangyuhua applicationofrecombinanttechnologyinproteininvestigations AT muleyavictor applicationofrecombinanttechnologyinproteininvestigations AT irvinghelenr applicationofrecombinanttechnologyinproteininvestigations |