Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1
Background: A number of receptor kinases contain guanylate cyclase (GC) catalytic centres encapsulated in the cytosolic kinase domain. A prototypical example is the phytosulfokine receptor 1 (PSKR1) that is involved in regulating growth responses in plants. PSKR1 contains both kinase and GC activiti...
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| Format: | Article |
| Language: | English |
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BioMed Central
2016
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| Online Access: | https://biosignaling.biomedcentral.com/articles/10.1186/s12964-014-0060-z http://hdl.handle.net/11408/1679 |
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| author | Muleya, Victor Wheeler, Janet I. Ruzvidzo, Oziniel Freihat, Lubna Manallack, David T. Gehring, Chris Irving, Helen R. |
| author_facet | Muleya, Victor Wheeler, Janet I. Ruzvidzo, Oziniel Freihat, Lubna Manallack, David T. Gehring, Chris Irving, Helen R. |
| author_sort | Muleya, Victor |
| collection | DSpace |
| description | Background: A number of receptor kinases contain guanylate cyclase (GC) catalytic centres encapsulated in the cytosolic kinase domain. A prototypical example is the phytosulfokine receptor 1 (PSKR1) that is involved in regulating growth responses in plants. PSKR1 contains both kinase and GC activities however the underlying mechanisms regulating the dual functions have remained elusive.
Findings: Here, we confirm the dual activity of the cytoplasmic domain of the PSKR1 receptor. We show that mutations within the guanylate cyclase centre modulate the GC activity while not affecting the kinase catalytic activity. Using physiologically relevant Ca2+ levels, we demonstrate that its GC activity is enhanced over two-fold by Ca2+ in a concentration-dependent manner. Conversely, increasing Ca2+ levels inhibits kinase activity up to 500-fold at 100 nM Ca2+.
Conclusions: Changes in calcium at physiological levels can regulate the kinase and GC activities of PSKR1. We therefore propose a functional model of how calcium acts as a bimodal switch between kinase and GC activity in PSKR1 that could be relevant to other members of this novel class of ligand-activated receptor kinases. |
| format | Article |
| id | ir-11408-1679 |
| institution | My University |
| language | English |
| publishDate | 2016 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | ir-11408-16792022-10-15T20:06:29Z Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1 Muleya, Victor Wheeler, Janet I. Ruzvidzo, Oziniel Freihat, Lubna Manallack, David T. Gehring, Chris Irving, Helen R. Calcium, Guanylate cyclase, Kinase, PSKR1 Background: A number of receptor kinases contain guanylate cyclase (GC) catalytic centres encapsulated in the cytosolic kinase domain. A prototypical example is the phytosulfokine receptor 1 (PSKR1) that is involved in regulating growth responses in plants. PSKR1 contains both kinase and GC activities however the underlying mechanisms regulating the dual functions have remained elusive. Findings: Here, we confirm the dual activity of the cytoplasmic domain of the PSKR1 receptor. We show that mutations within the guanylate cyclase centre modulate the GC activity while not affecting the kinase catalytic activity. Using physiologically relevant Ca2+ levels, we demonstrate that its GC activity is enhanced over two-fold by Ca2+ in a concentration-dependent manner. Conversely, increasing Ca2+ levels inhibits kinase activity up to 500-fold at 100 nM Ca2+. Conclusions: Changes in calcium at physiological levels can regulate the kinase and GC activities of PSKR1. We therefore propose a functional model of how calcium acts as a bimodal switch between kinase and GC activity in PSKR1 that could be relevant to other members of this novel class of ligand-activated receptor kinases. 2016-07-13T13:33:49Z 2016-07-13T13:33:49Z 2014 Article 1478-811X https://biosignaling.biomedcentral.com/articles/10.1186/s12964-014-0060-z http://hdl.handle.net/11408/1679 en Cell Communication and Signaling; open BioMed Central |
| spellingShingle | Calcium, Guanylate cyclase, Kinase, PSKR1 Muleya, Victor Wheeler, Janet I. Ruzvidzo, Oziniel Freihat, Lubna Manallack, David T. Gehring, Chris Irving, Helen R. Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1 |
| title | Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1 |
| title_full | Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1 |
| title_fullStr | Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1 |
| title_full_unstemmed | Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1 |
| title_short | Calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1 |
| title_sort | calcium is the switch in the moonlighting dual function of the ligand-activated receptor kinase phytosulfokine receptor 1 |
| topic | Calcium, Guanylate cyclase, Kinase, PSKR1 |
| url | https://biosignaling.biomedcentral.com/articles/10.1186/s12964-014-0060-z http://hdl.handle.net/11408/1679 |
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