HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions
The change in electrochemical behavior of horseradish peroxidase (HRP) activity induced by trace metals was used as a basis for developing an amperometric biosensor. The HRP was immobilized on maize tassel-multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions. The FTIR and UV-Vis...
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IEEE
2016
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| Online Access: | http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6688375 http://hdl.handle.net/11408/1538 |
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| author | Moyo, Mambo Jonathan, Okonkwo O. Nana, Agyei M. |
| author_facet | Moyo, Mambo Jonathan, Okonkwo O. Nana, Agyei M. |
| author_sort | Moyo, Mambo |
| collection | DSpace |
| description | The change in electrochemical behavior of horseradish peroxidase (HRP) activity induced by trace metals was used as a basis for developing an amperometric biosensor. The HRP was immobilized on maize tassel-multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions. The FTIR and UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCNT composite. Using Cd2002B; as a model divalent metal ion, the inhibition rate was proportional to the concentration in the range from 0.002-0.030 mg L-1 with a limit of detection of 0.51 μg L-1. Representative Dixon and Cornish-Bowden plots showed that the reaction was reversible and noncompetitive. |
| format | Article |
| id | ir-11408-1538 |
| institution | My University |
| language | English |
| publishDate | 2016 |
| publisher | IEEE |
| record_format | dspace |
| spelling | ir-11408-15382022-06-27T13:49:06Z HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions Moyo, Mambo Jonathan, Okonkwo O. Nana, Agyei M. Horseradish peroxidase, Maize tassel, Multi walled carbon nanotubes, Amperometric biosensor, Healy metal, Inhibition The change in electrochemical behavior of horseradish peroxidase (HRP) activity induced by trace metals was used as a basis for developing an amperometric biosensor. The HRP was immobilized on maize tassel-multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions. The FTIR and UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCNT composite. Using Cd2002B; as a model divalent metal ion, the inhibition rate was proportional to the concentration in the range from 0.002-0.030 mg L-1 with a limit of detection of 0.51 μg L-1. Representative Dixon and Cornish-Bowden plots showed that the reaction was reversible and noncompetitive. 2016-06-09T10:48:38Z 2016-06-09T10:48:38Z 2013 Article 1930-0395 http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6688375 http://hdl.handle.net/11408/1538 en Sensors;p. 1 - 4 open IEEE |
| spellingShingle | Horseradish peroxidase, Maize tassel, Multi walled carbon nanotubes, Amperometric biosensor, Healy metal, Inhibition Moyo, Mambo Jonathan, Okonkwo O. Nana, Agyei M. HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions |
| title | HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions |
| title_full | HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions |
| title_fullStr | HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions |
| title_full_unstemmed | HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions |
| title_short | HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions |
| title_sort | hrp biosensor based on carbonized maize tassel-mwnts modified electrode for the detection of divalent trace metal ions |
| topic | Horseradish peroxidase, Maize tassel, Multi walled carbon nanotubes, Amperometric biosensor, Healy metal, Inhibition |
| url | http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6688375 http://hdl.handle.net/11408/1538 |
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