HRP biosensor based on carbonized maize tassel-MWNTs modified electrode for the detection of divalent trace metal ions
The change in electrochemical behavior of horseradish peroxidase (HRP) activity induced by trace metals was used as a basis for developing an amperometric biosensor. The HRP was immobilized on maize tassel-multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions. The FTIR and UV-Vis...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
IEEE
2016
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| Subjects: | |
| Online Access: | http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6688375 http://hdl.handle.net/11408/1538 |
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| Summary: | The change in electrochemical behavior of horseradish peroxidase (HRP) activity induced by trace metals was used as a basis for developing an amperometric biosensor. The HRP was immobilized on maize tassel-multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions. The FTIR and UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCNT composite. Using Cd2002B; as a model divalent metal ion, the inhibition rate was proportional to the concentration in the range from 0.002-0.030 mg L-1 with a limit of detection of 0.51 μg L-1. Representative Dixon and Cornish-Bowden plots showed that the reaction was reversible and noncompetitive. |
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