A novel hydrogen peroxide biosensor based on adsorption of horseradish peroxidase onto a nanobiomaterial composite modified glassy carbon electrode.

In, this work, a novel hydrogen peroxide biosensor derived from maize-tassel (MT) and multiwalled carbon nanotube (MWCNT) composite was used to adsorb horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. Tile morphology and structure of the p...

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Bibliographic Details
Main Authors: Moyo, Mambo, Okonkwo, Jonathan, Agyei, Nana
Format: Article
Language:en_US
Published: Wiley-VCH Vcrlag GmbH & Co. 2016
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Online Access:http://hdl.handle.net/11408/1175
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Summary:In, this work, a novel hydrogen peroxide biosensor derived from maize-tassel (MT) and multiwalled carbon nanotube (MWCNT) composite was used to adsorb horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. Tile morphology and structure of the products were characterized by SEM, FTIR and UV-visible spectroscopy. The electrochemical and electrocatalytic performance of the HRP/MT- MWCNT/GCE was studied using voltammetrie and amperometric methods. The amperometric response of the biosensor varied linearly with concentration of H20; from 9 pM to 1 inM with detection limit of 4.0 pM (S/N = 3).Furthermore, the biosensor exhibited good reproducibility and stability.