Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics
The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on tradition...
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| Format: | Article |
| Language: | English |
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Elsevier
2022
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| Online Access: | https://doi.org/10.1016/j.ab.2020.113762 http://hdl.handle.net/11408/5173 |
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| author | Mukama, Omar Nie, Chengrong Habimana, Jean de Dieu Meng, Xiaogao Ting, Yuan Songwe, Fanuel Farga, Ammar Al Mugisha, Samson Rwibasira, Peter Zhang, Yinghui Zeng, Lingwen |
| author_facet | Mukama, Omar Nie, Chengrong Habimana, Jean de Dieu Meng, Xiaogao Ting, Yuan Songwe, Fanuel Farga, Ammar Al Mugisha, Samson Rwibasira, Peter Zhang, Yinghui Zeng, Lingwen |
| author_sort | Mukama, Omar |
| collection | DSpace |
| description | The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on traditional methods such as gel electrophoresis, colorimetry, turbidity, which could be non-specific and inconvenient. Thus, this review will first elaborate various isothermal amplification techniques (principle, merits, and demerits) and their potentials when combined with lateral flow approach for point-of-care nucleic acid diagnostics. Different methods for monitoring carryover contamination resulting from amplification product contamination will be discussed. Then, we will present recent advances in diagnostics with both target pre-amplification and CRISPR-Cas systems, which exhibit collateral cleavage of target nucleic acid and a reporter single strand nucleic acid within the vicinity. When the reporter is fluorophore-labeled, it provides a detectable signal by fluorescence or lateral flow biosensors. Lastly, we will discuss how CRISPR-Cas system based diagnostics could be more effective, affordable and portable for on-site detection. |
| format | Article |
| id | ir-11408-5173 |
| institution | My University |
| language | English |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | ir-11408-51732022-09-30T06:48:55Z Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics Mukama, Omar Nie, Chengrong Habimana, Jean de Dieu Meng, Xiaogao Ting, Yuan Songwe, Fanuel Farga, Ammar Al Mugisha, Samson Rwibasira, Peter Zhang, Yinghui Zeng, Lingwen Isothermal amplification Nucleic acids detection Lateral flow biosensor CRISPR-Cas system diagnostics The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on traditional methods such as gel electrophoresis, colorimetry, turbidity, which could be non-specific and inconvenient. Thus, this review will first elaborate various isothermal amplification techniques (principle, merits, and demerits) and their potentials when combined with lateral flow approach for point-of-care nucleic acid diagnostics. Different methods for monitoring carryover contamination resulting from amplification product contamination will be discussed. Then, we will present recent advances in diagnostics with both target pre-amplification and CRISPR-Cas systems, which exhibit collateral cleavage of target nucleic acid and a reporter single strand nucleic acid within the vicinity. When the reporter is fluorophore-labeled, it provides a detectable signal by fluorescence or lateral flow biosensors. Lastly, we will discuss how CRISPR-Cas system based diagnostics could be more effective, affordable and portable for on-site detection. 2022-09-30T06:48:55Z 2022-09-30T06:48:55Z 2020-05-05 Article 0003-2697 https://doi.org/10.1016/j.ab.2020.113762 http://hdl.handle.net/11408/5173 en Analytical Biochemistry;Vol. 600, No. 113762 open Elsevier |
| spellingShingle | Isothermal amplification Nucleic acids detection Lateral flow biosensor CRISPR-Cas system diagnostics Mukama, Omar Nie, Chengrong Habimana, Jean de Dieu Meng, Xiaogao Ting, Yuan Songwe, Fanuel Farga, Ammar Al Mugisha, Samson Rwibasira, Peter Zhang, Yinghui Zeng, Lingwen Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics |
| title | Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics |
| title_full | Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics |
| title_fullStr | Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics |
| title_full_unstemmed | Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics |
| title_short | Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics |
| title_sort | synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics |
| topic | Isothermal amplification Nucleic acids detection Lateral flow biosensor CRISPR-Cas system diagnostics |
| url | https://doi.org/10.1016/j.ab.2020.113762 http://hdl.handle.net/11408/5173 |
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