An amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution
A biosensor for trace metal ions based on horseradish peroxidase (HRP) immobilized on maize tassel- multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions is described herein. The biosensor was characterized using Fourier transform infrared (FTIR), UV-vis spectrometry, voltammetri...
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Elsevier Inc.
2016
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| Online Access: | http://hdl.handle.net/11408/1195 |
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| author | Moyo, Mambo Okonkwo, Jonathan Agyei, Nana |
| author_facet | Moyo, Mambo Okonkwo, Jonathan Agyei, Nana |
| author_sort | Moyo, Mambo |
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| description | A biosensor for trace metal ions based on horseradish peroxidase (HRP) immobilized on maize tassel- multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions is described herein. The biosensor was characterized using Fourier transform infrared (FTIR), UV-vis spectrometry, voltammetric and
amperometric methods. The FTIR and UV-vis results inferred that HRP was not denatured during its immobilization on MT-MWCNT composite. The biosensing principle was based on the determination of the cathodic responses of the immobilized HRP to H202, before and after incubation in trace metal standard
solutions. Under optimum conditions, the inhibition rates of trace metals were proportional to their concentrations in the range cf 0.092-0.55mgL-1, 0.068-2mgL~'for Pb2* and Cu2* respectively. The limits of detection were 2.5p.gL~' for Pb2* and 4.2p,gL_1 for Cu2*. Representative Dixon and Cornish-Bowden plots were used to deduce the mode of inhibition induced by the trace metal ions. The inhibition was reversible and mixed for both metal ions. Furthermore, the biosensor showed good stability, selectivity, repeatability and reproducibility. |
| format | Article |
| id | ir-11408-1195 |
| institution | My University |
| language | English |
| publishDate | 2016 |
| publisher | Elsevier Inc. |
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| spelling | ir-11408-11952022-06-27T13:49:06Z An amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution Moyo, Mambo Okonkwo, Jonathan Agyei, Nana Horseradish peroxidase, maize tassel, multi walled carbon nanotubes, amperometric biosensor, heavy metal ions, inhibitory studies. A biosensor for trace metal ions based on horseradish peroxidase (HRP) immobilized on maize tassel- multiwalled carbon nanotube (MT-MWCNT) through electrostatic interactions is described herein. The biosensor was characterized using Fourier transform infrared (FTIR), UV-vis spectrometry, voltammetric and amperometric methods. The FTIR and UV-vis results inferred that HRP was not denatured during its immobilization on MT-MWCNT composite. The biosensing principle was based on the determination of the cathodic responses of the immobilized HRP to H202, before and after incubation in trace metal standard solutions. Under optimum conditions, the inhibition rates of trace metals were proportional to their concentrations in the range cf 0.092-0.55mgL-1, 0.068-2mgL~'for Pb2* and Cu2* respectively. The limits of detection were 2.5p.gL~' for Pb2* and 4.2p,gL_1 for Cu2*. Representative Dixon and Cornish-Bowden plots were used to deduce the mode of inhibition induced by the trace metal ions. The inhibition was reversible and mixed for both metal ions. Furthermore, the biosensor showed good stability, selectivity, repeatability and reproducibility. 2016-05-04T10:52:03Z 2016-05-04T10:52:03Z 2014 Article http://hdl.handle.net/11408/1195 en Enzyme and Microbiology Technology;Vol.56; p28-34. open Elsevier Inc. |
| spellingShingle | Horseradish peroxidase, maize tassel, multi walled carbon nanotubes, amperometric biosensor, heavy metal ions, inhibitory studies. Moyo, Mambo Okonkwo, Jonathan Agyei, Nana An amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution |
| title | An amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution |
| title_full | An amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution |
| title_fullStr | An amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution |
| title_full_unstemmed | An amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution |
| title_short | An amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution |
| title_sort | amperometric biosensor based on horseradish peroxidase immobilized onto maize tassel-multi-walled carbon nanotubes modified glassy carbon electrode for determination of heavy metal ions in aqueous solution |
| topic | Horseradish peroxidase, maize tassel, multi walled carbon nanotubes, amperometric biosensor, heavy metal ions, inhibitory studies. |
| url | http://hdl.handle.net/11408/1195 |
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