Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection
The present work is focused on the application of a hydrogen peroxide (H2O2 ) biosensor for the detection of heavy metals ions. The biosensor was constructed by physical adsorption of horseradish peroxidase (HRP) onto a maize tassel (MT)-multiwalled carbon nanotube (MWCNT) composite and was characte...
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2016
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| Online Access: | http://hdl.handle.net/11408/1153 |
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| author | Moyo, Mambo Okonkwo, Jonathan |
| author_facet | Moyo, Mambo Okonkwo, Jonathan |
| author_sort | Moyo, Mambo |
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| description | The present work is focused on the application of a hydrogen peroxide (H2O2 ) biosensor for the detection of heavy metals ions. The biosensor was constructed by physical adsorption of horseradish peroxidase (HRP) onto a maize tassel (MT)-multiwalled carbon nanotube (MWCNT) composite and was characterized using spectroscopic and voltammetric methods, UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCn t composite. The biosensing principle was based on the determination of the cathodic responses of the immobilized HRP to H2O2 . before and after incubation in trace metal standard solutions. Using Cd2* as a model metal ion, the inhibition rate of the trace metal was proportional to concentration in the range of 2 -3 0 p.gL_1 with a limit of detection of 0.51 p,gL_ l. Representative Dixon and Cornish-Bowden plots showed that the reaction was non-competitive. The developed biosensor exhibited good stability,repeatability and reproducibility, thus providing a new promising tool for analysis of enzyme
inhibitors. |
| format | Article |
| id | ir-11408-1153 |
| institution | My University |
| language | English |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | ir-11408-11532022-06-27T13:49:06Z Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection Moyo, Mambo Okonkwo, Jonathan Horseradish peroxidase, biosensor, maize tassel, multi-walled carbon nanotubes,Cd2* ,inhibitory analysis. The present work is focused on the application of a hydrogen peroxide (H2O2 ) biosensor for the detection of heavy metals ions. The biosensor was constructed by physical adsorption of horseradish peroxidase (HRP) onto a maize tassel (MT)-multiwalled carbon nanotube (MWCNT) composite and was characterized using spectroscopic and voltammetric methods, UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCn t composite. The biosensing principle was based on the determination of the cathodic responses of the immobilized HRP to H2O2 . before and after incubation in trace metal standard solutions. Using Cd2* as a model metal ion, the inhibition rate of the trace metal was proportional to concentration in the range of 2 -3 0 p.gL_1 with a limit of detection of 0.51 p,gL_ l. Representative Dixon and Cornish-Bowden plots showed that the reaction was non-competitive. The developed biosensor exhibited good stability,repeatability and reproducibility, thus providing a new promising tool for analysis of enzyme inhibitors. 2016-04-29T14:38:57Z 2016-04-29T14:38:57Z 2013 Article 0925-4005 http://hdl.handle.net/11408/1153 en Sensors and Actuators B: Chemical;p.515-521 open Elsevier |
| spellingShingle | Horseradish peroxidase, biosensor, maize tassel, multi-walled carbon nanotubes,Cd2* ,inhibitory analysis. Moyo, Mambo Okonkwo, Jonathan Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection |
| title | Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection |
| title_full | Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection |
| title_fullStr | Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection |
| title_full_unstemmed | Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection |
| title_short | Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection |
| title_sort | horseradish peroxidase biosensor based on maize tassel-mwcnts composite for cadmium detection |
| topic | Horseradish peroxidase, biosensor, maize tassel, multi-walled carbon nanotubes,Cd2* ,inhibitory analysis. |
| url | http://hdl.handle.net/11408/1153 |
| work_keys_str_mv | AT moyomambo horseradishperoxidasebiosensorbasedonmaizetasselmwcntscompositeforcadmiumdetection AT okonkwojonathan horseradishperoxidasebiosensorbasedonmaizetasselmwcntscompositeforcadmiumdetection |