Horseradish peroxidase biosensor based on maize tassel-MWCNTs composite for cadmium detection
The present work is focused on the application of a hydrogen peroxide (H2O2 ) biosensor for the detection of heavy metals ions. The biosensor was constructed by physical adsorption of horseradish peroxidase (HRP) onto a maize tassel (MT)-multiwalled carbon nanotube (MWCNT) composite and was characte...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016
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| Subjects: | |
| Online Access: | http://hdl.handle.net/11408/1153 |
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| Summary: | The present work is focused on the application of a hydrogen peroxide (H2O2 ) biosensor for the detection of heavy metals ions. The biosensor was constructed by physical adsorption of horseradish peroxidase (HRP) onto a maize tassel (MT)-multiwalled carbon nanotube (MWCNT) composite and was characterized using spectroscopic and voltammetric methods, UV-Vis results inferred that HRP was not denatured during its immobilization on MT-MWCn t composite. The biosensing principle was based on the determination of the cathodic responses of the immobilized HRP to H2O2 . before and after incubation in trace metal standard solutions. Using Cd2* as a model metal ion, the inhibition rate of the trace metal was proportional to concentration in the range of 2 -3 0 p.gL_1 with a limit of detection of 0.51 p,gL_ l. Representative Dixon and Cornish-Bowden plots showed that the reaction was non-competitive. The developed biosensor exhibited good stability,repeatability and reproducibility, thus providing a new promising tool for analysis of enzyme
inhibitors. |
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